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Der mikroskopiebasierte Assay zur Untersuchung und Analyse der Recycling-Endosomen mittels SNARE-Trafficking
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JoVE 신문 생물학
The Microscopy-Based Assay to Study and Analyze the Recycling Endosomes using SNARE Trafficking
DOI:

08:51 min

February 12, 2022

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Chapters

  • 00:06Introduction
  • 01:04Seeding of Mouse Melanocytes on Pre-Treated Coverslips
  • 01:42Transfection of Cells with the STX13 Plasmids
  • 02:39Fixation of the Cells
  • 03:11Immunostaining of the Cells
  • 05:05Results: Quantification of Overlap Between the RE Localized Proteins and Melanosomes
  • 07:25Results: Quantification of Recycling Endosomes’ Tublar Number and Length
  • 07:56Conclusion

Summary

자동 번역

Recycling-Endosomen sind Teil des endosomalen tubulären Netzwerks. Hier stellen wir eine Methode zur Quantifizierung der Dynamik von Recycling-Endosomen mit GFP-STX13 als Organellenmarker vor.

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