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The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
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JoVE 신문 생화학
The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination
DOI:

04:50 min

February 08, 2022

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Chapters

  • 00:00Introduction
  • 00:30Glycogen Extraction
  • 02:44Results: The Effect of Sucrose Concentration on the Particle Size of Glycogen
  • 04:25Conclusion

Summary

자동 번역

An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial.

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