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Chapters
- 00:04Introduction
- 00:52Sample Collection, Processing, and Analysis
- 03:12Degradation of Hydrocarbons
- 05:04Genomic DNA Isolation of the Pure Culture
- 06:4916S rRNA Sequencing
- 08:07Results: Isolation and Characterization of Hydrocarbon Degrading Bacteria from Aquatic Habitats
- 09:11Conclusion
We present the process of isolating, propagating, and characterizing hydrocarbon-degrading bacteria from aquatic habitats. The protocol outlines bacterial isolation, identification by the 16S rRNA method, and testing of their hydrocarbon-degrading potential. This article would help researchers in characterizing microbial biodiversity in environmental samples, and specifically screen for microbes with bioremediation potential.
