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Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes
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환경과학
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Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes

DOI:
10.3791/63109-v

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07:42 min

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January 10, 2022

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, , , , ,
1Biodiversity Unit,University of Turku, 2Department of Biology,University of Turku, 3Nutrition and Health Unit,Eurecat Technology Centre of Catalonia, 4Department of Biochemistry and Biotechnology,Rovira i Virgili University

Chapters

  • 00:05Introduction
  • 00:52Two Field Experiments to Test the Effect of Glyphosate Based Products (GBPs)
  • 01:58Microbiome Analyses: 16S rRNA and ITS Gene Amplification, and Library Preparation
  • 04:59Algorithm to Determine the Potential Sensitivity of Organisms to Glyphosate
  • 05:56Assessing the EPSPS Class from Universal Microbial Markers
  • 06:40Results: Quantification of Microbial Species Sensitivity after GBPs Usage
  • 07:10Conclusion

Summary

자동 번역

자동 번역

Glyphosate-based products (GBP) are the most common broad-spectrum herbicides worldwide. In this article, we introduce general guidelines to quantify the effect of GBP on microbiomes, from field experiments to bioinformatics analyses.

Tags

Keywords: GlyphosateMicrobiome16S RRNAExperimental DesignPlant SamplesQuail BeddingPCR AmplificationDNA ExtractionBioinformatics Workflow

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