Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements <em>In Vitro</em>

Natalija Bogunovic; Karlijn B. Rombouts; Kak Khee Yeung

doi:10.3791/63122

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Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

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Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

DOI:

10.3791/63122-v

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08:28 min

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February 15, 2022

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Natalija Bogunovic*^1,2,3, Karlijn B. Rombouts*^1,2, Kak Khee Yeung²

¹Department of Vascular Surgery, Amsterdam UMC,Vrije Universiteit Amsterdam, ²Department of Physiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC,Vrije Universiteit Amsterdam, ³Laboratory of Experimental Cardiology, Department of Cardiology,Leiden University Medical Center

Chapters

00:04Introduction
00:45Isolating Primary Human Smooth Muscle Cells (SMCs) from Aortic Biopsies
02:40Isolating Primary Dermal Fibroblasts from Skin Biopsies
04:25Measuring Contraction
06:00Results: Measurement of Aortic SMC Contraction upon Ionomycin Stimulation and SMC Marker Expression
07:51Conclusion

Summary

자동 번역

This paper describes an explant culture-based method for the isolation and culturing of primary, patient-specific human aortic smooth muscle cells and dermal fibroblasts. Furthermore, a novel method is presented for measuring cell contraction and subsequent analysis, which can be used to study patient-specific differences in these cells.

Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

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Isolation of Primary Patient-specific Aortic Smooth Muscle Cells and Semiquantitative Real-time Contraction Measurements In Vitro

•

•

•

Chapters

Summary

자동 번역

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

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