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Aislamiento de células endoteliales retinianas murinas para secuenciación de próxima generación
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JoVE 신문 발생학
Isolation of Murine Retinal Endothelial Cells for Next-Generation Sequencing
DOI:

09:59 min

October 11, 2021

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Chapters

  • 00:04Introduction
  • 00:38Obtain Mouse Eyes for Retinal Isolation and Isolate Mouse Retinal Tissue
  • 02:30Digest Retinal Tissue into a Single Cell Suspension and Immunostain the Cells with Antibodies
  • 04:07Prepare for Fluorescence‐Activated Cell Sorting
  • 05:19Isolate Viable Endothelial Cells via FACS
  • 07:18Results: Analysis of Isolated Endothelial Cells and Next‐Generation Sequencing After Isolation
  • 09:03Conclusion

Summary

자동 번역

Este protocolo describe un método para el aislamiento de células endoteliales retinianas postnatales murinas optimizadas para el rendimiento, la pureza y la viabilidad celular. Estas células son adecuadas para los enfoques de secuenciación de próxima generación.

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