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Chapters
- 00:04Introduction
- 00:33SU8 Mold Fabrication
- 03:51PDMS Chip Fabrication
- 06:43PDMS Membrane Priming
- 08:00Results: Cell Lineage Study and Neuron Development Using Long-Term Growth and Imaging Device
- 10:04Conclusion
The protocol describes a simple microfluidic chip design and microfabrication methodology used to grow C. elegans in presence of a continuous food supply for up to 36 h. The growth and imaging device also enables intermittent long-term high-resolution imaging of cellular and sub-cellular processes during development for several days.
