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Isoleer celtypespecifieke RNA's uit snap-frozen heterogene weefselmonsters zonder celsortering
JoVE 신문
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JoVE 신문 생물학
Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting
DOI:

08:30 min

December 08, 2021

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Chapters

  • 00:05Introduction
  • 00:43Tissue Lysis and Homogenization
  • 01:31Immunoprecipitation
  • 02:30RNA Extraction
  • 05:31Results: Microarray and qPCR Analysis of TRAP Samples
  • 07:45Conclusion

Summary

자동 번역

Dit protocol is bedoeld om celtypespecifiek te isoleren, ribosomale mRNA's te vertalen met behulp van het NuTRAP-muismodel.

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