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Developmental Biology

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JoVE Journal Developmental Biology
Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5
 

Murine Neural Plate Targeting by In Utero Nano-Injection (NEPTUNE) at Embryonic Day 7.5

Article DOI: 10.3791/63148-v • 10:49 min • February 14th, 2022
February 14th, 2022 •
1, 1, 1, 1
1Department of Cell and Molecular Biology, Karolinska Institutet

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요약
Automatic Translation

In this protocol, we describe how to inject the mouse amniotic cavity at E7.5 with lentivirus, leading to uniform transduction of the entire neural plate, with minimal detrimental effects on survival or embryonic development.

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Keywords: NEPTUNE Murine Neural Plate Targeting In Utero Nano-injection Embryonic Day 7.5 Genetic Manipulation Knockdown Lineage Tracing ShRNA CdRNA Barcoded Viral Libraries Neuroscience Organ Systems Amniotic Cavity Ultrasound Glass Needle Metal Plunger Capillary Needle Oil Solution Anesthetized Female Mouse Painkiller Chlorhexidine Solution Skin Incision Muscle Layer Uterine Horns PBS Elastic Membrane
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