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Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse

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Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse

Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse

DOI:

10.3791/63154-v

•

05:30 min

•

March 09, 2022

•

Anthony G. Giacalone, Matthew E. Merritt, Mukundan Ragavan

¹Department of Biochemistry and Molecular Biology,University of Florida

Chapters

00:04Introduction
01:01Celiotomy
01:44Cannulation of Portal Vein
02:50Resection of Liver Post Portal Vein Cannulation
04:18Results: Liver Function Assessment by Oxygen Consumption and Flow Rate Measurement
04:45Conclusion

Summary

자동 번역

The protocol describes a straightforward method of resectioning an intact mouse liver for metabolic studies through portal vein perfusion.

Tags

Keywords: Ex Vivo Hepatic Perfusion Portal Vein Mouse Liver Metabolism Disease Models NAFLD NASH Pharmaceutical Assessment Anesthesia Celiotomy Portal Vein Cannulation Catheter Suture Syringe

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