Journal
/
유전학
/
Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

DOI:
10.3791/63177-v

•

10:08 min

•

December 21, 2021

•

, ,
1Laboratory of Genetics,University of Wisconsin-Madison

Chapters

  • 00:05Introduction
  • 00:56Synthesis of Guide RNAs
  • 02:03Microinjection of CRISPR-Cas9 Cocktail into a One-Cell Zebrafish Embryo to Generate Maternal Crispants
  • 03:07Screening for Somatic INDELs in F0 Injected Embryos
  • 04:54Identification of Maternal-Effect Phenotypes in Maternal Crispant Embryos
  • 06:21Sequencing Alleles in Maternal Crispant Haploids
  • 08:18Results: Identification of Maternal Effect Phenotypes in a Rapid and Resource Efficient Manner
  • 09:08Conclusion

Summary

자동 번역

자동 번역

Early development is dependent on maternally-inherited products, and the role of many of these products is currently unknown. Herein, we described a protocol that uses CRISPR-Cas9 to identify maternal-effect phenotypes in a single generation.

Tags

Keywords: Maternal CRISPRMaternal Effect GenesEarly DevelopmentEmbryogenesisGuide RNAIn-vitro TranscriptionZebrafishEmbryo Injection

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/63177?article_type=v

The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
check_url/kr/63177?article_type=v

Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks
check_url/kr/63177?article_type=v

Preparation of rAAV9 to Overexpress or Knockdown Genes in Mouse Hearts
check_url/kr/63177?article_type=v

Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
check_url/kr/63177?article_type=v

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
check_url/kr/63177?article_type=v

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
check_url/kr/63177?article_type=v

CRISPR-Mediated Reorganization of Chromatin Loop Structure
check_url/kr/63177?article_type=v

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
check_url/kr/63177?article_type=v

qKAT: Quantitative Semi-automated Typing of Killer-cell Immunoglobulin-like Receptor Genes
check_url/kr/63177?article_type=v

Determining the Egg Fertilization Rate of Bemisia tabaci Using a Cytogenetic Technique

Read Article