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Chapters
- 00:05Introduction
- 00:56Synthesis of Guide RNAs
- 02:03Microinjection of CRISPR-Cas9 Cocktail into a One-Cell Zebrafish Embryo to Generate Maternal Crispants
- 03:07Screening for Somatic INDELs in F0 Injected Embryos
- 04:54Identification of Maternal-Effect Phenotypes in Maternal Crispant Embryos
- 06:21Sequencing Alleles in Maternal Crispant Haploids
- 08:18Results: Identification of Maternal Effect Phenotypes in a Rapid and Resource Efficient Manner
- 09:08Conclusion
