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量子点偶联SARS-CoV-2穗状三聚体的高通量共聚焦成像，以追踪HEK293T细胞中的结合和内吞作用

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High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells

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量子点偶联SARS-CoV-2穗状三聚体的高通量共聚焦成像，以追踪HEK293T细胞中的结合和内吞作用

DOI:

10.3791/63202-v

•

06:39 min

•

April 21, 2022

•

Bruce Nguyen Tran, Eunkeu Oh, Kimihiro Susumu³, Mason Wolak, Kirill Gorshkov

¹National Center for Advancing Translational Sciences, ²Optical Sciences Division,Code 5600, Naval Research Laboratory, ³Jacobs Corporation

Chapters

00:04Introduction
00:30Treatment of Cells with QD-Spike
01:39Fixation and Nuclei Staining
02:36Acquisition Set-Up and Imaging
04:39Results: Imaging and Visualization of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers
06:11Conclusion

Summary

자동 번역

在该协议中，与重组SARS-CoV-2尖峰偶联的量子点使基于细胞的测定能够监测质膜上与hACE2的尖峰结合以及随后结合蛋白进入细胞质的内吞作用。

Tags

Quantum Dot SARS-CoV-2 Spike Trimer Binding Endocytosis HEK293T Cells High-throughput Confocal Imaging Virus Entry Cell Recognition Quantum Dot Conjugation BSA Dilution PFA Nuclear Dye Imaging Protocol Microscopy

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