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1Leslie Dan Faculty of Pharmacy,University of Toronto, 2Laboratory of Virology and Experimental Therapy (LAVITE), Department of Virology, Aggeu Magalhães Institute (IAM),Oswaldo Cruz Foundation (Fiocruz), 3Department of Biomedical Engineering,Boston University, 4Molecular Biology, Cell Biology & Biochemistry Program, Graduate School of Arts and Sciences,Boston University, 5Department of Mechanical and Industrial Engineering,University of Toronto
Chapters
- 00:04Introduction
- 01:07Computational Design of Toehold Switches
- 02:45Construction of Toehold Switches by PCR
- 03:32Initial Screening of the Switches
- 05:16NASBA Primer Screening and Sensitivity
- 06:55RT-qPCR for Zika Virus Detection
- 08:08Results: Evaluation of Toehold Switches for Paper-Based Zika Sensors
- 10:01Conclusion
Tillgång till decentraliserad, billig och högkapacitetsdiagnostik som kan distribueras i samhället för decentraliserad testning är avgörande för att bekämpa globala hälsokriser. Detta manuskript beskriver hur man bygger pappersbaserad diagnostik för virala RNA-sekvenser som kan detekteras med en bärbar optisk läsare.
