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ACT1-CUP1测定确定出芽酵母中剪接体突变体的底物特异性敏感性

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ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

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ACT1-CUP1测定确定出芽酵母中剪接体突变体的底物特异性敏感性

DOI:

10.3791/63232-v

•

07:31 min

•

June 30, 2022

•

Clarisse van der Feltz

¹College of Arts and Sciences,Northwest University

Chapters

00:05Introduction
00:47Pouring Plates for the ACT1-CUP1 Assay
02:13ACT1-CUP1 Plate Preparation
03:26Plating the Strains on the Copper Plates, Data Collection, and Analysis
05:09Results: Impact of Mutant Splicing Factors on the Spliceosomal Function
06:50Conclusion

Summary

자동 번역

ACT1-CUP1测定是一种铜生长测定，可快速读取前体信使RNA（pre-mRNA）剪接以及突变剪接因子对剪接体功能的影响。本研究提供了一个协议，并强调了解决感兴趣的拼接问题的定制可能。

Tags

Keywords: ACT1-CUP1 Assay Pre-messenger RNA Splicing Splicing Factor Mutation Growth Phenotype Leucine Dropout Growth Media Copper Sulfate Optical Density Spectrophotometer Dilution Bunsen Burner Replicator

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