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면역학 및 감염병학
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Kemisk kartografi tilgange til undersøgelse af trypanosomatidinfektion
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Chemical Cartography Approaches to Study Trypanosomatid Infection

Kemisk kartografi tilgange til undersøgelse af trypanosomatidinfektion

DOI:
10.3791/63255-v

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08:21 min

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January 21, 2022

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, , ,
1Department of Chemistry and Biochemistry,University of Oklahoma, Norman, 2Laboratories of Molecular Anthropology and Microbiome Research,University of Oklahoma, Norman, 3Department of Anthropology,University of Oklahoma, Norman, 4Department of Microbiology and Plant Biology,University of Oklahoma, Norman

Chapters

  • 00:04Introduction
  • 00:55Tissue Collection and Metabolite Extraction
  • 02:563D Model Generation
  • 04:55‘ili Plot Generation
  • 07:04Results: 3D Model of Metabolite Distribution During Trypanosomatid Infection
  • 07:37Conclusion

Summary

자동 번역

자동 번역

Denne protokol beskriver trinene til at generere en 3D-model af metabolitfordeling under trypanosomatidinfektion, herunder prøveindsamling, metabolitekstraktion, en oversigt over væskekromatografi-tandemmassespektrometri dataindsamling, 3D-modelgenerering og endelig datavisualisering.

Tags

Chemical Cartography3D ModelingMetabolite DistributionParasite DistributionCross-organ MappingMetabolomicsWater-based HomogenizationOrganic ExtractionMass SpectrometrySketchUp SoftwareOrgan MappingPathogenesisTrypanosomatid Infection

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