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JoVE Journal Biology
Funktionel vurdering af kinesin-7 CENP-E i spermatocytter ved anvendelse af in vivo-hæmning , immunofluorescens og flowcytometri
 
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Funktionel vurdering af kinesin-7 CENP-E i spermatocytter ved anvendelse af in vivo-hæmning , immunofluorescens og flowcytometri

Article DOI: 10.3791/63271-v • 09:41 min • December 28th, 2021
December 28th, 2021 •
1,2, 1,2, 3,4, 1,2, 1,2, 1,2, 1,2, 1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University

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Denne artikel rapporterer en in vivo hæmning af CENP-E gennem abdominal kirurgi og testikel injektion af GSK923295, en værdifuld model for mandlig meiotisk deling. Ved hjælp af immunofluorescens-, flowcytometri- og transmissionselektronmikroskopi-assays viser vi, at CENP-E-hæmning resulterer i kromosomforskydning og genomustabilitet i musespermatocytter.

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Denne måned i JoVE udgave 178 kinesin spermatocyt meiose CENP-E spindel kromosom mikrotubuli
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