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1Department of Cell Biology and Genetics, The School of Basic Medical Sciences,Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine,Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital,Affiliated Hospital of Fujian Medical University
Chapters
- 00:05Introduction
- 01:13Construction of GSK923295-Mediated Centromere Protein E (CENP-E) Inhibition Mouse Models
- 02:18Hematoxylin-eosin (HE) Staining and Histopathology
- 03:57Immunofluorescence and Confocal Microscopy
- 05:31Flow Cytometry
- 07:04Results: Analysis of Seminiferous Tubules and the Disruption of Spermatogenesis Post CENP-E Inhibition by GSK923295
- 08:59Conclusion
Denna artikel rapporterar en in vivo-hämning av CENP-E genom bukkirurgi och testikelinjektion av GSK923295, en värdefull modell för manlig meiotisk delning. Med hjälp av immunofluorescens-, flödescytometri- och transmissionselektronmikroskopianalyser visar vi att CENP-E-hämning resulterar i kromosomfelinriktning och genominstabilitet i musspermatocyter.
