JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 01:19Equipment Setup and DiI Perfusion
- 04:08Preparation of Footpad Tissue for Confocal Laser Scanning Microscopy
- 05:01Confocal Laser Scanning Microscopy
- 06:37Results: Anatomy of the Murine Hindlimb Vasculature
- 07:20Conclusion
The present protocol describes a unique, clinically relevant model of peripheral arterial disease that combines femoral artery and vein electrocoagulation with the administration of a nitric oxide synthase inhibitor to induce hindlimb gangrene in FVB mice. Intracardiac DiI perfusion is then used for high-resolution, three-dimensional imaging of the footpad vasculature.
