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小鼠后肢坏死模型中脚垫脉管系统的高分辨率三维成像
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High-Resolution Three-Dimensional Imaging of the Footpad Vasculature in a Murine Hindlimb Gangrene Model
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小鼠后肢坏死模型中脚垫脉管系统的高分辨率三维成像

DOI:
10.3791/63284-v

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08:16 min

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March 16, 2022

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, , , , , , , ,
1Dewitt Daughtry Family Department of Surgery,University of Miami Miller School of Medicine, 2Vascular Biology Institute,University of Miami Miller School of Medicine, 3University of Miami, 4Analytical Imaging Core Facility,University of Miami

Chapters

  • 00:04Introduction
  • 01:19Equipment Setup and DiI Perfusion
  • 04:08Preparation of Footpad Tissue for Confocal Laser Scanning Microscopy
  • 05:01Confocal Laser Scanning Microscopy
  • 06:37Results: Anatomy of the Murine Hindlimb Vasculature
  • 07:20Conclusion

Summary

자동 번역

자동 번역

本方案描述了一种独特的、临床相关的外周动脉疾病模型，该模型将股动脉和静脉电凝与施用一氧化氮合酶抑制剂相结合，以诱导FVB小鼠的后肢坏疽。然后，心内DiI灌注用于脚垫脉管系统的高分辨率三维成像。

Tags

Keywords: 3D ImagingFoot PadVasculatureMurine HindlimbGangrene ModelDiI PerfusionNeovascularizationPeripheral Arterial DiseaseCritical Limb IschemiaPreclinical Research

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