Journal
/
발생학
/
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

DOI:
10.3791/63298-v

•

10:12 min

•

December 16, 2021

•

,
1College of Health and Life Sciences,Hamad Bin Khalifa University (HBKU), Qatar Foundation, 2Diabetes Research Center, Qatar Biomedical Research Institute (QBRI),Hamad Bin Khalifa University (HBKU), Qatar Foundation (QF)

Chapters

  • 00:05Introduction
  • 01:22Induction of Definitive Endoderm Differentiation in Human Pluripotent Stem Cells (hPSCs) (Stage 1)
  • 02:10Immunofluorescence Analysis of hPSC-Derived Definitive Endoderm (Stage 1)
  • 04:19Generation of the Primitive Gut Tube from hPSCs (Stage 2)
  • 05:50Assessment of Differentiation Efficiency of Generating Pancreatic Progenitors
  • 08:10Results: Comparison between Optimized and Non-Optimized Protocol for Scalable Generation of Pancreatic Progenitors from hPSCs
  • 09:21Conclusion

Summary

자동 번역

자동 번역

The present protocol describes an enhanced method to increase the co-expression of PDX1 and NKX6.1 transcription factors in pancreatic progenitors derived from human pluripotent stem cells (hPSCs) in planar monolayers. This is achieved by replenishing the fresh matrix, manipulating cell density, and dissociating the endodermal cells.

Tags

Human Pluripotent Stem CellsPancreatic Beta-cell Precursors2D Culture SystemCell TherapyDiabetesPancreatic DevelopmentDefinitive EndodermDifferentiationImmunocytochemistryCHIR-99021

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/63298?article_type=v

Culturing Human Pluripotent and Neural Stem Cells in an Enclosed Cell Culture System for Basic and Preclinical Research
check_url/kr/63298?article_type=v

Large-Scale Production of Cardiomyocytes from Human Pluripotent Stem Cells Using a Highly Reproducible Small Molecule-Based Differentiation Protocol
check_url/kr/63298?article_type=v

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
check_url/kr/63298?article_type=v

Efficient Differentiation of Pluripotent Stem Cells to NKX6-1+ Pancreatic Progenitors
check_url/kr/63298?article_type=v

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells
check_url/kr/63298?article_type=v

In Vitro Differentiation of Human Mesenchymal Stem Cells into Functional Cardiomyocyte-like Cells
check_url/kr/63298?article_type=v

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
check_url/kr/63298?article_type=v

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells
check_url/kr/63298?article_type=v

Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
check_url/kr/63298?article_type=v

Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells

Read Article