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1Laboratory of Biophysics of Synaptic Processes,Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, 2Department of Radiophotonics and Microwave Technologies,Kazan National Research Technical University named after A.N. Tupolev, 3Department of Normal Physiology,Kazan State Medical University
Chapters
- 00:05Introduction
- 01:12Dye Loading Procedure
- 03:28Video Capture with Confocal Microscopy
- 06:14Video Processing
- 07:56Video Analysis
- 09:55Results: Mouse Nerve Terminals Loaded with the Ca2+ Indicator and Representative Trace of Calcium Signal
- 10:18Conclusion
El protocolo describe el método de carga de un tinte de calcio fluorescente a través del nervio cortado en las terminales nerviosas motoras del ratón. Además, se presenta un método único para registrar transitorios rápidos de calcio en las terminaciones nerviosas periféricas mediante microscopía confocal.
