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Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)
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Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy (3D-SIM)

DOI:
10.3791/63327-v

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07:40 min

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February 11, 2022

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, ,
1Minerva Foundation Institute for Medical Research, 2Department of Computer Science,Aalto University School of Science, 3HiLIFE – Neuroscience Center,University of Helsinki, 4HiLIFE – Institute of Biotechnology,University of Helsinki

Chapters

  • 00:04Introduction
  • 00:49Sample Preparation
  • 02:57Imaging
  • 04:32Image Analysis
  • 06:06Results: 3D-Structured Illumination Microscopy Image Analysis
  • 06:51Conclusion

Summary

자동 번역

자동 번역

The present protocol describes a method to visualize and measure actin rings and other components of the membrane periodic skeleton of the axon initial segment using cultured rat hippocampal neurons and 3D-structured illumination microscopy (3D-SIM).

Tags

Keywords: 3D-SIMMembrane Periodic SkeletonAxon Initial SegmentFluorescence MicroscopySample PreparationAnkyrin GFluoro 4FerodinMountingImmersion Oil

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