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Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
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JoVE 신문 암 연구학
Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
DOI:

08:15 min

February 03, 2022

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Chapters

  • 00:05Introduction
  • 01:03Probing Mitochondrial Energy Metabolism of Single Spheroids Using XF Technology
  • 02:44Preparation of XF Assay Medium
  • 03:08Preparation of the Sensor Cartridge
  • 04:25Seeding Single Spheroids into the Spheroid Assay Microplate
  • 05:04Preparation and Loading of Compounds into the Sensor Cartridge for XF Assays
  • 05:31Results: Mitochondrial Energy Metabolism in 3D Cancer Cell-Line-Derived Spheroids
  • 07:35Conclusion

Summary

자동 번역

These protocols will help users probe mitochondrial energy metabolism in 3D cancer cell-line-derived spheroids using Seahorse extracellular flux analysis.

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