JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04Introduction
- 01:01Environment Setup and RiboCode Installation
- 01:23Data Preparation
- 03:35Removing Ribosomal RNA Contamination
- 03:59Aligning the Clean Reads to the Genome
- 04:25Size Selection of Ribosome Protected Fragments, Identification of P-Sites, and De novo Annotate Translating Open Reading Frames
- 04:59Results: Identification of the Novel Open Reding Frames (ORFs) with the Application of RiboCode
- 07:20Conclusion
Translating ribosomes decode three nucleotides per codon into peptides. Their movement along mRNA, captured by ribosome profiling, produces the footprints exhibiting characteristic triplet periodicity. This protocol describes how to use RiboCode to decipher this prominent feature from ribosome profiling data to identify actively translated open reading frames at the whole-transcriptome level.
