Journal
/
암 연구학
/
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

Generation and Expansion of Primary, Malignant Pleural Mesothelioma Tumor Lines

DOI:
10.3791/63374-v

•

08:01 min

•

April 21, 2022

•

, ,
1Department of Translational Molecular Pathology,University of Texas MD Anderson Cancer Center

Chapters

  • 00:05Introduction
  • 00:45Digestion of Tumor Tissue
  • 03:16Generation of Primary Tumor Cell Line
  • 04:47Characterization and Banking of Established Primary Tumor Cell Line
  • 06:13Results: Determination of Fibroblast Contamination of Cell Culture and Flow Cytometry Surface Staining of Cell Lines
  • 07:12Conclusion

Summary

자동 번역

자동 번역

The goal of this method paper is to demonstrate a robust and reproducible methodology for the enrichment, generation, and expansion of primary tumor cell lines from surgically resected pleural mesothelioma.

Tags

Primary Malignant Pleural MesotheliomaTumor Cell ExpansionIn Vitro ModelTumor Digesting Enzymatic CocktailTumor DissociationCell StrainerTumor Digestion MediumCell CultureFibroblast Contamination

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Analysis of Side Population in Solid Tumor Cell Lines

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish

Circulating Tumor Cell Lines: an Innovative Tool for Fundamental and Translational Research

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells

Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Read Article