JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:04introduction
- 00:55Generation and Surgical Implantation of the Mammary Imaging Window (MIW)
- 04:13Positioning and Maintaining Mouse on the Microscope Stage for Imaging
- 05:52Set Up for 4D, Intensity-Based, Label-Free Intravital Imaging of Dynamic Cell Behavior
- 08:02Fluorescence Lifetime Imaging (FLIM) of NAD(P)H
- 08:43Results: Intravital Imaging of the Mammary Tumor Microenvironment
- 10:15Conclusion
De hier beschreven intravitale beeldvormingsmethode maakt gebruik van collageen tweede harmonische generatie en endogene fluorescentie van de metabole co-factor NAD (P) H om een niet-invasieve tumormicro-omgeving te segmenteren in tumor-, stromale en vasculaire compartimenten voor diepgaande analyse van 4D intravitale beelden.
