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1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio),Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering,National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans),Unigranrio, 4Post-graduation Program in Biotechnology,National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School,Fluminense Federal Fluminense
Chapters
- 00:05Introduction
- 00:55Trypsinization of ASC Monolayer at Passage Three
- 01:45Micro Molded Non-Adherent Agarose Hydrogel Fabrication
- 02:31Bio-Fabrication of ASC Spheroids Using the Automated Pipetting System
- 05:48Results: Automated Pipette System for Large-Scale Generation of ASC Spheroids
- 07:17Conclusion
Ici, nous décrivons la production à grande échelle de sphéroïdes stromaux / cellules souches (ASC) dérivés des adipeux à l’aide d’un système de pipetage automatisé pour ensemencer la suspension cellulaire, assurant ainsi l’homogénéité de la taille et de la forme des sphéroïdes. Ces sphéroïdes ASC peuvent être utilisés comme blocs de construction pour les approches de bio-impression 3D.
Tags
Here Are The Key Keywords Extracted From The Given Text:3D BioprintingAdipose-derived Stem CellsSpheroidsAutomated ProductionMicrobiology ContaminationLabor HoursSoftware ParametersCell Culture Media3D Cell CultureStem Cell SuspensionASC PelletDMEM-LowFBSMicromolded AgaroseUltra Pure AgaroseSilicone Mold12-well Plastic PlateLaminar FlowCarbon DioxideLabWare EditorVirtual Work TablePipettesTip BoxesPlastic TubesPlates
