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1Nucleus of Multidisciplinary Research in Biology (Numpex-Bio),Federal University of Rio de Janeiro, 2Laboratory of Tissue Bioengineering,National Institute of Metrology, Quality and Technology (Inmetro), 3Post-graduation Program of Translational Biomedicine (Biotrans),Unigranrio, 4Post-graduation Program in Biotechnology,National Institute of Metrology, Quality and Technology (Inmetro), 5Dental School,Fluminense Federal Fluminense
Chapters
- 00:05Introduction
- 00:55Trypsinization of ASC Monolayer at Passage Three
- 01:45Micro Molded Non-Adherent Agarose Hydrogel Fabrication
- 02:31Bio-Fabrication of ASC Spheroids Using the Automated Pipetting System
- 05:48Results: Automated Pipette System for Large-Scale Generation of ASC Spheroids
- 07:17Conclusion
Her beskriver vi storskala produksjon av fettavledet stromal/ stamcelle (ASC) sfæroider ved hjelp av et automatisert pipetteringssystem for å frø cellesuspensjonen, og dermed sikre homogenitet av sfæroid størrelse og form. Disse ASC-sfæroidene kan brukes som byggesteiner for 3D-bioprintingsmetoder.
Tags
Here Are The Key Keywords Extracted From The Given Text:
3D BioprintingAdipose-derived Stem CellsSpheroidsAutomated ProductionMicrobiology ContaminationLabor HoursSoftware ParametersCell Culture Media3D Cell CultureStem Cell SuspensionASC PelletDMEM-LowFBSMicromolded AgaroseUltra Pure AgaroseSilicone Mold12-well Plastic PlateLaminar FlowCarbon DioxideLabWare EditorVirtual Work TablePipettesTip BoxesPlastic TubesPlates
