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Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

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Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

DOI:

10.3791/63438-v

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08:04 min

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January 31, 2022

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Savina R. Cammalleri^2,3, Jessica Knox³, Peter J. Roy^3,4

¹Department of Molecular Genetics,University of Toronto, ²Department of Biochemistry,University of Toronto, ³The Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ⁴Department of Pharmacology and Toxicology,University of Toronto

Chapters

00:05Introduction
00:38Culturing of D. dipsaci
03:22Extraction and Collection of D. dipsaci
04:14In Vitro Small Molecule Screen
05:36Data Collection and Analysis
06:13Results: In Vitro Small Molecule Screen Assay for Candidate Nematicides
07:40Conclusion

Summary

자동 번역

The present protocol describes a reliable and straightforward method for culturing, collecting, and screening Ditylenchus dipsaci.

Tags

Keywords: Plant Parasitic Nematode Ditylenchus Dipsaci Nematicides Culturing Medium-throughput Chemical Screens Nutrient Agar Gamborg B5 Media Seed Sterilization Pea Seeds Sucrose Solution

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