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Chapters
- 00:04Introduction
- 00:58Dissection and Isolation of Mouse Cerebral Arterioles
- 02:30Digestion of Parenchymal Arterioles and Preparation of Endothelial Tubes
- 03:47Utilization of Arteriolar Endothelial Tubes for the Examination of Cellular Physiology and Cellular Imaging
- 04:54Results: Assessment of Endothelial Function
- 05:50Conclusion
Intensive preparation of intact mouse cerebral endothelial "tubes" from cerebral parenchymal arterioles is illustrated for studying cerebral blood flow regulation. Further, we demonstrate the experimental strengths of this endothelial study model for fluorescence imaging and electrophysiology measurement of key cellular signaling pathways, including changes in intracellular [Ca2+] and membrane potential.
