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Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
JoVE 신문
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JoVE 신문 생화학
Deciphering Molecular Mechanism of Histone Assembly by DNA Curtain Technique
DOI:

06:32 min

March 09, 2022

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Chapters

  • 00:00Introduction
  • 00:28Preparation of the Flow Cell
  • 02:16Connecting Flow Cell to the Microfluidic System and Loading on Microscope
  • 02:49Histone Assembly by Abo1 Using DNA Curtain
  • 03:46Results: Visualization of Histone Loading by Abo1 Using Single‐Molecule DNA Curtain
  • 05:46Conclusion

Summary

자동 번역

DNA curtain, a high-throughput single-molecule imaging technique, provides a platform for real-time visualization of diverse protein-DNA interactions. The present protocol utilizes the DNA curtain technique to investigate the biological role and molecular mechanism of Abo1, a Schizosaccharomyces pombe bromodomain-containing AAA+ ATPase.

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