Journal
/
/
有机溶剂基蛋白质沉淀,在质谱分析之前实现强大的蛋白质组纯化
JoVE 신문
화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 화학
Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry
DOI:

11:12 min

February 07, 2022

, , , , ,

Chapters

  • 00:04Introduction
  • 00:55Rapid (Vial-Based) Protein Precipitation with Acetone
  • 02:27Protein Precipitation by Chloroform/Methanol/Water (CMW)
  • 04:30Protein Precipitation Using a Disposable Filtration Cartridge
  • 05:45Resolubilization of Protein Pellet with Formic Acid
  • 06:58Solid-Phase Extraction (SPE) Clean-Up
  • 08:20Results: Protein Precipitation via Vial-Based Versus Cartridge Approach
  • 10:22Conclusion

Summary

자동 번역

本方案描述了在受控条件下的溶剂型蛋白质沉淀,以便在质谱分析之前稳定快速地回收和纯化蛋白质组样品。

Related Videos

Read Article