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通过磁活化细胞分选在脱髓鞘动物模型中分离小鼠原代小胶质细胞

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Isolation of Mouse Primary Microglia by Magnetic-Activated Cell Sorting in Animal Models of Demyelination

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通过磁活化细胞分选在脱髓鞘动物模型中分离小鼠原代小胶质细胞

DOI:

10.3791/63511-v

•

04:53 min

•

April 05, 2022

•

Hang Zhang, Sheng Yang, Man Chen, Dai-Shi Tian, Chuan Qin

¹Department of Neurology, Tongji Hospital, Tongji Medical College,Huazhong University of Science and Technology

Chapters

00:04Introduction
00:42Mice Dissection and Tissue Dissociation
01:35Debris Removal and Magnetic Separation of Microglial Cells
03:26Results: Gating Strategy Used in Flow Cytometry Analysis Before and After MACS and Gating Strategy for Living/Dead Single Cells After MACS
04:27Conclusion

Summary

자동 번역

在这里，我们提出了一种方案，利用柱状磁激活细胞分选在脱髓鞘疾病的动物模型中分离和纯化原代小胶质细胞。

Tags

Mouse Primary Microglia Magnetic-activated Cell Sorting Demyelination Corpus Callosum Enzyme Digestion CD11b Beads Positive Selection Magnetic Field

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