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Spændingsmåler tether prober til kvantificering af vækstfaktor medieret integrin mekanik og vedhæftning
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Tension Gauge Tether Probes for Quantifying Growth Factor Mediated Integrin Mechanics and Adhesion

Spændingsmåler tether prober til kvantificering af vækstfaktor medieret integrin mekanik og vedhæftning

DOI:
10.3791/63529-v

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09:56 min

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February 11, 2022

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1Department of Cell, Developmental, and Integrative Biology,University of Alabama at Birmingham

Chapters

  • 00:04Introduction
  • 00:47Surface Preparation
  • 04:25Cell Preparation and Staining
  • 06:28Image Acquisition
  • 07:43Results: Effect of Ligand-Activated EGFR on Integrin-Mediated Cell Mechanics and Adhesion Formation in Cos-7 Cells
  • 09:11Conclusion

Summary

자동 번역

자동 번역

TGT overflade er en innovativ platform til at studere vækstfaktor-integrin crosstalk. Det fleksible sondedesign, vedhæftningsligandens specificitet og præcise modulering af stimuleringsbetingelser muliggør robuste kvantitative vurderinger af EGFR-integrin-samspil. Resultaterne fremhæver EGFR som en 'mechano-organizer' tuning integrin mekanik, der påvirker fokal vedhæftningssamling og cellespredning.

Tags

Tension Gauge TetherIntegrin MechanicsCell AdhesionEGFR-Integrin CrosstalkSurface FunctionalizationAPTES SilanizationNHS-BiotinCell MechanicsBioengineering Protocols

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