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使用靶标下的超低输入切割在染色质上的单细胞因子定位,并使用核酸酶释放
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생물학
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JoVE 신문 생물학
Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
DOI:

09:20 min

February 01, 2022

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Chapters

  • 00:04Introduction
  • 01:17Pre-Blocking of Samples to Prevent Early Digestion by MNase
  • 01:46Addition of Primary Antibody
  • 03:09Addition of pA-MNase or pA/G-MNase
  • 04:04Directed DNA Digestion
  • 05:04Sample Fractionation and DNA Extraction
  • 07:09Results: Protein Localization on Chromatin Using Single Cell uliCUT&RUN
  • 08:54Conclusion

Summary

자동 번역

CUT&RUN及其变体可用于确定染色质上的蛋白质占用率。该协议描述了如何使用单细胞uliCUT&RUN确定染色质上的蛋白质定位。

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