Journal
/
암 연구학
/
Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis
JoVE 신문
암 연구학
Author Produced
This content is Free Access.
JoVE 신문 암 연구학
Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

Freeze-Fracture Electron Microscopy for Extracellular Vesicle Analysis

DOI:
10.3791/63550-v

•

11:30 min

•

September 16, 2022

•

, , ,
1Faculty of Medicine, Institute of Cell Biology,University of Ljubljana

Chapters

  • 00:05Introduction
  • 02:01Culturing Cancer Urothelial Cells and Isolation of EVs
  • 06:19Fracturing of EVs and Making Replicas
  • 08:25Cleaning Replicas and Replica Analysis
  • 09:47Results
  • 10:54Conclusion

Summary

자동 번역

자동 번역

We present a protocol for the isolation and freeze-fracturing of extracellular vesicles (EVs) originating from cancerous urothelial cells. The freeze-fracture technique revealed the EVs' diameter and shape and-as a unique feature-the internal organization of the EV membranes. These are of immense importance in understanding how EVs interact with the recipient membranes.

Tags

Keywords: Extracellular VesiclesFreeze-fractureElectron MicroscopyMicrovesiclesMembrane CompositionCell-cell Communication

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/63550?article_type=v

A Preclinical Mouse Model of Osteosarcoma to Define the Extracellular Vesicle-mediated Communication Between Tumor and Mesenchymal Stem Cells
check_url/kr/63550?article_type=v

Simple and Rapid Method to Obtain High-quality Tumor DNA from Clinical-pathological Specimens Using Touch Imprint Cytology
check_url/kr/63550?article_type=v

Phospho Flow Cytometry with Fluorescent Cell Barcoding for Single Cell Signaling Analysis and Biomarker Discovery
check_url/kr/63550?article_type=v

Extraction of Extracellular Vesicles from Whole Tissue
check_url/kr/63550?article_type=v

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
check_url/kr/63550?article_type=v

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels
check_url/kr/63550?article_type=v

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells
check_url/kr/63550?article_type=v

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis
check_url/kr/63550?article_type=v

Fluorescence Microscopy for ATP Internalization Mediated by Macropinocytosis in Human Tumor Cells and Tumor-xenografted Mice
check_url/kr/63550?article_type=v

In Vivo Immunogenicity Screening of Tumor-Derived Extracellular Vesicles by Flow Cytometry of Splenic T Cells

Read Article