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使用Sigma的提取物N -安培组织PCR试剂盒小鼠组织的快速基因分型
JoVE 신문
생물학
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JoVE 신문 생물학
Rapid Genotyping of Mouse Tissue Using Sigma’s Extract-N-Amp Tissue PCR Kit
DOI:

12:39 min

January 22, 2008

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Chapters

  • 00:00Title
  • 00:12Introduction
  • 01:04Preparing Tissue and PCR Reaction Mixture
  • 06:58Real Time Quantitative PCR
  • 11:10Melting Curve Analysis
  • 11:45Conclusion

Summary

자동 번역

鼠尾样品的完整的基因分型,包括组织消化和PCR读数,是在一个和一个半小时使用Sigma的SYBR绿色提取N -安培组织PCR试剂盒。

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