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斑马鱼角膜伤口愈合：从磨损到伤口闭合成像分析

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Zebrafish Corneal Wound Healing: From Abrasion to Wound Closure Imaging Analysis

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斑马鱼角膜伤口愈合：从磨损到伤口闭合成像分析

DOI:

10.3791/63605-v

•

08:19 min

•

March 01, 2022

•

Kaisa Ikkala, Sini Raatikainen, Frederic Michon²

¹Institute of Biotechnology, HiLIFE,University of Helsinki, ²Institute for Neurosciences of Montpellier,Univ Montpellier

Chapters

00:04Introduction
01:01Preparation and Sample Collection
03:03Sample Processing for Electron Microscopy
04:25Imaging and Measuring Cell Shape, Size, and Microridge Pattern
06:36Results: Imaging Analysis Using Scanning Electron Microscopy
07:28Conclusion

Summary

자동 번역

该协议的重点是通过磨损破坏斑马鱼的眼表面，以评估细胞水平上随后的伤口闭合。这种方法利用眼毛刺来部分去除角膜上皮，并使用扫描电子显微镜来跟踪伤口闭合过程中细胞形态的变化。

Tags

Zebrafish Corneal Wound Healing Corneal Abrasion Wound Closure Corneal Transparency Pathogenesis Ophthalmic Burr Tricaine Anesthesia Analgesic Glutaraldehyde Sodium Phosphate Buffer

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