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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

DOI:
10.3791/63606-v

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08:12 min

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May 05, 2022

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, , , , , , , , , , ,
1Department of Biochemistry,Albert Einstein College of Medicine

Chapters

  • 00:04Introduction
  • 00:53Preparation of the 3D Culture System
  • 02:20Growth of Spheroids in the Bioreactor
  • 03:15Histone Extraction
  • 04:27The First Round of Derivatization
  • 05:03Desalting, Sample Cleanup, and Sample Preparation for LC-MS/MS
  • 06:08Results: Identification and Quantification of Histone Post-Translational Modifications
  • 07:37Conclusion

Summary

자동 번역

자동 번역

This protocol outlines how a three-dimensional cell culture system can be used to model, treat, and analyze chromatin modifications in a near-physiological state.

Tags

3D Cell CultureHistone Post-translational ModificationsLiquid Chromatography Mass SpectrometrySpheroid FormationBioreactor

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