Journal
/
/
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
DOI:

10:07 min

March 16, 2022

, , , , , , ,

Chapters

  • 00:04Introduction
  • 00:43Microinjection and Culture of the Eggs
  • 04:01Mutation Rate Estimation and Screening of Mutants
  • 06:08Establishment and Passaging of Mutant Lines and Egg Cryopreservation and Resuscitatio
  • 07:48Results: CRISPR/Cas9 Ribonuclease-Mediated Construction of Homozygous Locust Mutants
  • 09:19Conclusion

Summary

자동 번역

This study provides a systematically optimized procedure of CRISPR/Cas9 ribonuclease-based construction of homozygous locust mutants as well as a detailed method for cryopreservation and resuscitation of the locust eggs.

Related Videos

Read Article