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白色念珠菌中转录因子-DNA结合相互作用的全基因组分析：全面的CUT&RUN方法和数据分析工作流程
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Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
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白色念珠菌中转录因子-DNA结合相互作用的全基因组分析：全面的CUT&RUN方法和数据分析工作流程

DOI:
10.3791/63655-v

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07:48 min

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April 01, 2022

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, , , , , ,
1Department of Molecular and Cell Biology,University of California, Merced, 2Quantitative and Systems Biology Graduate Program,University of California, Merced, 3Department of Applied Mathematics,University of California, Merced, 4Health Sciences Research Institute,University of California, Merced

Chapters

  • 00:04Introduction
  • 00:49Isolation of Nuclei and Primary Antibody Binding
  • 02:40Binding of pAG-MNase to Antibody
  • 03:36Library Preparation for Sequencing
  • 05:22CUT&RUN Sequence Analysis
  • 05:54Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
  • 07:22Conclusion

Summary

자동 번역

자동 번역

该协议描述了一种实验方法和数据分析工作流程，用于在靶标下切割并使用核酸酶（CUT&RUN）在人类真菌病原体 白色念珠菌中释放。

Tags

Genome-wide ProfilingTranscription Factor-DNA BindingCandida AlbicansCUT&RUNChIP-chipChIP-seqEpitope TaggingNuclei IsolationFluorescence MicroscopyAntibody IncubationProtein AG-MNaseData Analysis Workflow

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