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硝基还原酶/甲硝唑介导的消融和MATLAB平台（RpEGEN）研究斑马鱼视网膜色素上皮的再生

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Nitroreductase/Metronidazole-Mediated Ablation and a MATLAB Platform (RpEGEN) for Studying Regeneration of the Zebrafish Retinal Pigment Epithelium

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硝基还原酶/甲硝唑介导的消融和MATLAB平台（RpEGEN）研究斑马鱼视网膜色素上皮的再生

DOI:

10.3791/63658-v

•

13:12 min

•

March 02, 2022

•

Lyndsay L. Leach, G. Burch Fisher, Jeffrey M. Gross³

¹Department of Ophthalmology, Louis J. Fox Center for Vision Restoration,University of Pittsburgh School of Medicine, ²Earth Research Institute,University of California, Santa Barbara, ³Department of Developmental Biology,University of Pittsburgh School of Medicine

Chapters

00:05Introduction
01:00Screening Zebrafish Larvae for rpe65a:nfsB-eGFP and Genetic Ablation of the Retinal Pigment Epithelium
03:02Incorporating Pharmacological Treatment into Zebrafish Retinal Pigment Epithelium Ablation Protocol
06:35Confocal Microscope Z-stack Image Preprocessing in FIJI (ImageJ)
08:19Quantification and Visualization of RPE Regeneration Using RpEGEN Scripts
10:42Results: RPE Expression, Ablation, Regeneration Quantification
12:39Conclusion

Summary

자동 번역

该协议描述了使用转基因斑马鱼模型对视网膜色素上皮（RPE）进行遗传消融的方法。使用药理学化合物调整方案以纳入信号通路调节被广泛详细。开发了一种基于色素沉着的量化RPE再生的MATLAB平台，并进行了介绍和讨论。

Tags

Keywords: Zebrafish Retinal Pigment Epithelium RPE Regeneration Nitroreductase Metronidazole MATLAB RpEGEN Ablation Transgenic EGFP PTU Pharmacological Treatment

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