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Detectie van Wolbachia Stam wAlbB in Aedes albopictus cellijnen
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines
DOI:

08:08 min

June 01, 2022

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Chapters

  • 00:04Introduction
  • 00:43Cell Culture and DNA Extraction
  • 01:55Detection of Wolbachia Nucleic Acid and Quantitative PCR Analysis
  • 02:53Protein Extraction
  • 04:26Immunofluorescence Localization of Wolbachia Protein
  • 06:05Results: Detection of Intracellular Wolbachia Infection at the Nucleic acid and Protein Levels
  • 07:21Conclusion

Summary

자동 번역

Vier methoden werden gebruikt om intracellulaire Wolbachia te detecteren, die elkaar aanvulden en de detectienauwkeurigheid van Wolbachia-infectie van Aedes albopictus-afgeleide Aa23 en Aa23-T genezen van inheemse Wolbachia-infectie met behulp van antibiotica verbeterden.

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