Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

Sohee Choi; Jessica A. San Juan; Marie C. Heffern; Michael J. Stevenson

doi:10.3791/63668

Journal

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생화학

/

Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

DOI:

10.3791/63668-v

•

11:38 min

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April 05, 2022

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Sohee Choi, Jessica A. San Juan, Marie C. Heffern, Michael J. Stevenson

¹Department of Chemistry,University of San Francisco, ²Department of Chemistry,University of California, Davis

Chapters

00:04Introduction
00:59Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
02:44Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
03:56Isothermal Titration Calorimetry
09:00Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
10:48Conclusion

Summary

자동 번역

This article focuses on the use of electronic absorption spectroscopy and isothermal titration calorimetry to probe and quantify the thermodynamics of Cu(II) binding to peptides and proteins.

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Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores

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Chapters

Summary

자동 번역

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