Journal
/
/
発色団の存在下および非存在下におけるCu(II)とペプチド残基間の結合相互作用の定量化
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
DOI:

11:38 min

April 05, 2022

, , ,

Chapters

  • 00:04Introduction
  • 00:59Electronic Absorption Spectroscopy: Direct Titration with Buffer Competition
  • 02:44Electronic Absorption Spectroscopy: Peptide Competition with Chromophoric Ligand
  • 03:56Isothermal Titration Calorimetry
  • 09:00Results: Quantifying the Thermodynamics of Cu(II) Binding to Peptides and Proteins
  • 10:48Conclusion

Summary

자동 번역

この記事では、電子吸収分光法と等温滴定熱量測定を使用して、ペプチドおよびタンパク質に結合するCu(II)の熱力学をプローブおよび定量することに焦点を当てます。

Related Videos

Read Article