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Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

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Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

Probing Myosin Ensemble Mechanics in Actin Filament Bundles Using Optical Tweezers

DOI:

10.3791/63672-v

•

06:53 min

•

May 04, 2022

•

Omayma Al Azzam*¹, Janie C. Watts*¹, Justin E. Reynolds, Juliana E. Davis, Dana N. Reinemann²

¹Department of Chemical Engineering,University of Mississippi, ²Department of Biomedical Engineering,University of Mississippi

Chapters

00:04Introduction
00:44Flow Cell Preparation
01:25Actomyosin Bundle Preparation
02:30Force Measurements Using Optical Trap
05:45Results: Measuring Myosin Ensemble Force Generation Using Optical Tweezers
06:23Conclusion

Summary

자동 번역

Formation of actomyosin bundles in vitro and measuring myosin ensemble force generation using optical tweezers is presented and discussed.

Tags

Keywords: Optical Tweezers Actin Filament Bundles Myosin Ensemble Mechanics Cytoskeleton PLL-coated Coverslip Rhodamine Actin Casein Solution Biotinylated Actin Bead Suspension Laser Power

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