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透明质酸35 kDa对肠源单层早产小肠损伤体 外 模型及愈合的影响
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Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
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透明质酸35 kDa对肠源单层早产小肠损伤体 外 模型及愈合的影响

DOI:
10.3791/63758-v

•

09:36 min

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July 28, 2022

•

, , ,
1Department of Pediatrics, Division of Neonatal-Perinatal Medicine,University of Oklahoma Health Sciences Center

Chapters

  • 00:04Introduction
  • 00:46Enteroid Monolayer Formation
  • 03:47Monolayer Treatment and Scratch Wound Assay
  • 06:36Scratch Wound Analysis
  • 07:38Results: Enteroid Generation and Enteroid-Derived Monolayer Wound Healing Assay Procedure, Images, Analysis, and the Effect of HA35 on Wound Healing
  • 08:58Conclusion

Summary

자동 번역

자동 번역

该协议描述了一种在从非人灵长类动物回肠分离的三维（3D）肠衍生的二维（2D）单层上建立和执行划痕伤口测定的方法。

Tags

Hyaluronic AcidPretermSmall IntestineEnteroidMonolayerWound HealingRegenerationIn Vitro ModelCell CultureExtracellular MatrixCell DissociationTrypsinDMEM/F-122D Model

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