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用于检测人类精子功能缺陷的生物标志物的流式细胞术分析
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JoVE 신문 의학
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
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用于检测人类精子功能缺陷的生物标志物的流式细胞术分析

DOI:
10.3791/63790-v

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08:48 min

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April 21, 2022

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, , , , , , , , ,
1Institute of Toxicology, College of Preventive Medicine,Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine,Army Medical University (Third Military Medical University)

Chapters

  • 00:04Introduction
  • 01:01Cell Preparation and Sperm Cells Counting Using Computer‐Aided Sperm Analysis (CASA)
  • 01:37Staining Sperm Cells
  • 04:52Flow Cytometer Setup
  • 07:10Results: Flow Cytometric Analysis of Sperm Function Markers for Detecting Human Sperm Functional Defect in a Single Cytometry
  • 08:22Conclusion

Summary

자동 번역

자동 번역

本方案提供了一种实用的解决方案，允许使用单个细胞仪测量人类精子中的细胞凋亡、线粒体膜电位和DNA损伤。

Tags

Flow CytometrySperm Functional BiomarkersSperm ApoptosisMitochondrial Membrane PotentialSperm Function AnalysisInfertility DiagnosisSemen AnalysisJC-1 ProbeCCCPAnnexin-V FITC PI Staining

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