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Chapters
- 00:05Introduction
- 00:50Preparation of Hydrogel Solution
- 02:32Illumination and Photocrosslinking of Hydrogels via an LED Array
- 04:20Setting Up and Drug Treatment of 3D, Matrix-Embedded Cultures
- 08:22Results: Effect of HA Based 3D Miniaturized Cultures on Matrix Stiffness and Integrin-Binding Peptides
- 10:24Conclusion
The present protocol describes an experimental platform to assess the effects of mechanical and biochemical cues on chemotherapeutic responses of patient-derived glioblastoma cells in 3D matrix-mimetic cultures using a custom-made UV illumination device facilitating high-throughput photocrosslinking of hydrogels with tunable mechanical features.
